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- Title
ISOLATION AND MORPHOLOGICAL IDENTIFICATION OF FUNGI FROM DETERIORATING MONUMENTS OF MADHYA PRADESH, INDIA.
- Authors
Pandya, Vanshree; Jain, Sudhir Kumar
- Abstract
Fungi are the most commonly occurring microbes found on the surfaces of stone. They have very simple nutritional requirements and can absorb organic compounds from the environment. Fungal hyphae penetrate surfaces and grow inside the soil. This investigation was carried out in order to determine the diverse community of fungi growing on these deteriorating monuments. For this study, 55 samples of stone were collected from 10 ancient sites which were deteriorating. The samples were collected by use of scalpels, brushes, tweezers and stored in UV sterilised poly bags. The isolation was done by using dilution plate method, plating technique and moist chamber method. During this study, a number of fungal species were isolated. These mixed colonies were then purified from the isolation plates and separately incubated at 28±2°C. For this, selective media SDA (Sabouraud Dextrose Agar) was used. In order to prevent contamination from bacterial colonies, Chloramphenicol was added. After isolation, identification was done. For the microscopic examination of fungi compound light microscope was used. Tease mount method was employed to observe the septation, conidia or spores. For the preparation of mount Lactophenol Cotton Blue was used. Twenty-One fungal species were isolated from 17 genera which are accounted in this study. The most frequently occurring fungal species are Alternaria sp, A. nidulans, A. fumigatus, A. flavus, A. terreus, A. niger, Bipolaris sp., Curvularia sp., Cladosporium sp., Chaetomium sp., Chrysosporium sp., Exserohilum sp., Fusarium sp., Penicillium sp., Phoma sp., Poecilomyces sp., Talaromyces sp., Trichoderma sp., Torula sp., Ulocladium sp. and Verticillium sp. From the selected sites, there were few sites like Raisen Fort Raisen, Rajwada Palace Indore, Bhimbetka caves Raisen, Islamnagar Fort Bhopal and Bir Singh Palace Datia showed the maximum number of fungal species. These were followed by Shaukat Mahal Bhopal, Sadar Manzil Bhopal, Siddhawat ghat Ujjain, Bharthari Caves Ujjain and Gohar Mahal Bhopal. From these sites, areas showing fungal abundance were chosen for the collection of samples. The bio-corrosion caused by these isolates will help to evaluate the damage caused and their extent of involvement. The data and results brought about from this study will help for future researches and analysis. Moreover, this study determines the vast fungal diversity found on the deteriorating surface of the monuments of Madhya Pradesh. This study will further help in implementing the preventive measures for the conservation of heritage monuments.
- Subjects
FUNGI; ORGANIC compounds; SOIL pollution
- Publication
Journal of Advanced Scientific Research, 2022, Vol 13, Issue 7, p72
- ISSN
0976-9595
- Publication type
Article
- DOI
10.55218/JASR.202213708