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- Title
Development of a Rapid LC-MS/MS Method for the Determination of Emerging Fusarium mycotoxins Enniatins and Beauvericin in Human Biological Fluids.
- Authors
Serrano, Ana Belén; Anna Laura Capriotti; Chiara Cavaliere; Piovesana, Susy; Samperi, Roberto; Ventura, Salvatore; Laganà, Aldo
- Abstract
A novel method for the simultaneous determination of enniatins A, A1, B and B1 and beauvericin, both in human urine and plasma samples, was developed and validated. The method consisted of a simple and easy pretreatment, specific for each matrix, followed by solid phase extraction (SPE) and detection by high performance liquid chromatography-tandem mass spectrometry with an electrospray ion source. The optimized SPE method was performed on graphitized carbon black cartridges after suitable dilution of the extracts, which allowed high mycotoxin absolute recoveries (76%-103%) and the removal of the major interferences from the matrix. The method was extensively evaluated for plasma and urine samples separately, providing satisfactory results in terms of linearity (R² of 0.991-0.999), process efficiency (>81%), trueness (recoveries between 85% and 120%), intra-day precision (relative standard deviation, RSD < 18%), inter-day precision (RSD < 21%) and method quantification limits (ranging between 20 ng·L-1 and 40 ng·L-1 in plasma and between 5 ng·L-1 and 20 ng·L-1 in urine). Finally, the highly sensitive validated method was applied to some urine and plasma samples from different donors.
- Subjects
BEAUVERICIN; ENNIATINS; FUSARIUM; MYCOTOXINS; HIGH performance liquid chromatography; MASS spectrometry
- Publication
Toxins, 2015, Vol 7, Issue 9, p3554
- ISSN
2072-6651
- Publication type
Article
- DOI
10.3390/toxins7093554