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- Title
Selection of Peptide Ligands Binding to Fibroblast Growth Factor Receptor 1.
- Authors
Fan, Hongkuan; Duan, Yanfen; Zhou, Hui; Li, Wei; Li, Fei; Guo, Lili; Roeske, Roger W.
- Abstract
Inappropriate expression of fibroblast growth factors (FGFs) or activation of FGF receptors (FGFRs) could contribute to several human angiogenic pathologies. In an attempt to design antagonists of FGF, we developed a screening procedure for identifying peptide ligands binding to FGFR1. To retain the natural conformation of FGFR1 during screening, we expressed recombinant FGFR1 on the surface of Sf9 insect cells. A 6-mer phage display peptide library was then screened on the cell surface and a group of hydrophobic peptide sequences were identified. Further experiments demonstrated that the phages displaying these sequences can specifically bind to FGFR1. The docking analysis suggests that the peptide ValTyrMetSerProPhe can specifically bind to the hydrophobic surface of FGFR1. The synthetic peptide Ac-ValTyrMetSerProPhe-NH[sub 2] can inhibit mitogenic activity of aFGF and has the potential to become a therapeutic agent as an aFGF antagonist.
- Subjects
FIBROBLAST growth factors; NEOVASCULARIZATION
- Publication
IUBMB Life, 2002, Vol 54, Issue 2, p67
- ISSN
1521-6543
- Publication type
Article
- DOI
10.1080/15216540214308