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- Title
Development of Hollow-Fiber Liquid-Phase Microextraction Method for Determination of Urinary trans, trans-Muconic Acid as a Biomarker of Benzene Exposure.
- Authors
Ghamari, Farhad; Bahrami, Abdulrahman; Yamini, Yadollah; Shahna, Farshid Ghorbani; Moghimbeigi, Abbas
- Abstract
For the first time, hollow-fiber liquid-phase microextraction combined with high-performance liquid chromatography-ultraviolet was used to extract trans, trans-muconic acid, in urine samples of workers who had been exposed to benzene. The parameters affecting the metabolite extraction were optimized as follows: the volume of sample solution was 11 mL with pH 2, liquid membrane containing dihexyl ether as the supporter, 15% (w/v) of trioctylphosphine oxide as the carrier, the time of extraction was 120 minutes, and stirring rate was 500 rpm. Organic phase impregnated in the pores of a hollow fiber was extracted into 24 µL solution of 0.05 mol L-1 Na2CO3 located inside the lumen of the fiber. Under optimized conditions, a high enrichment factor of 153-182 folds, relative recovery of 83%-92%, and detection limit of 0.001 µg mL-1 were obtained. The method was successfully applied to the analysis of ttMA in real urine samples.
- Subjects
URINALYSIS; MUCONIC acid; HOLLOW fibers; EXTRACTION (Chemistry); BIOMARKERS; INDUSTRIAL hygiene
- Publication
Analytical Chemistry Insights, 2016, Issue 11, p65
- ISSN
1177-3901
- Publication type
Article
- DOI
10.4137/ACI.S40177