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- Title
Carboxypeptidase-B from Bubalus bubalis pancreas: Purification, properties and MALDI-TOF monitored activation of proinsulin.
- Authors
Shahid Nadeem, Muhammad; Nazia Murtaza, Bibi; Ahmad, Habib
- Abstract
Carboxypeptidase-B (E.C 3.4.17.2) catalyzes the hydrolysis of peptides and esters at C-terminus of arginine and lysine residues. Our study describes the large scale purification, N-terminal sequence analysis and physiochemical properties of pancreatic enzyme from river buffalo (Bubalus bubalis). The enzyme was purified up to 71 folds by anion-exchange chromatography with 21% final recovery. Purified enzyme displayed two bands on SDS-PAGE with molecular weights of 9 kDa and 26 kDa respectively, the N-terminal sequence of later was EFLDKLDFYV. The enzyme has shown optimum activity at pH 9.0 and 40 °C. The KM, Kcat and Kcat/KM values of purified carboxypeptidase-B with Hippuryl-L-Arg are 30µM, 72sec and 2.4x105 M-1 sec-1 respectively. A computer based model for the structure of enzyme was proposed by chromatographic studies of component fragments and N-terminal sequence. The enzyme purified in the present study was free of carboxypeptidase A and endoprotease contamination. It was efficiently used in the processing of recombinant buffalo proinsulin, in combination with trypsin. Activation of proinsulin was monitored by MALDI-TOF analysis of peptides before and after the action of enzymes.
- Subjects
CARBOXYPEPTIDASES; WATER buffalo; PROINSULIN; PEPTIDES; C-terminal residues; N-terminal residues; ARGININE; ENZYME inhibitors; THERAPEUTICS
- Publication
Pakistan Journal of Pharmaceutical Sciences, 2013, Vol 26, Issue 5, p907
- ISSN
1011-601X
- Publication type
Article