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- Title
Cytotoxicity and genotoxicity of various types of endodontic sealers in Chinese hamster ovary (CHO-K1) cells.
- Authors
Mi-Jeong JEON; Hyunjung KO; Su-Jung SHIN; Miri KIM
- Abstract
This study aimed to evaluate the cytotoxicity and genotoxicity of five endodontic sealers (AH Plus, MTA Fillapex, Endoseal MTA, Sealapex, and Zinc oxide eugenol) in Chinese hamster ovary cells. Cytotoxicity was evaluated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay to check cell viability at 1, 3, and 7 days. Genotoxicity was assessed by cytokinesis-block micronucleus, single-cell gel electrophoresis, and γH2AX immunofluorescence assays. Cell viability of all endodontic sealers, except Endoseal MTA, on day 1 was less than 100%. Endoseal MTA showed the highest cell viability on day 7. AH Plus and Endoseal MTA showed less DNA damage than other sealers. After complete setting, AH Plus and Endoseal MTA showed low genotoxicity, which could reduce DNA damage in periapical cells, making them suitable as endodontic sealers.
- Subjects
PIT &; fissure sealants (Dentistry); CYTOTOXINS; CHO cell; GENETIC toxicology
- Publication
Dental Materials Journal, 2023, Vol 42, Issue 6, p774
- ISSN
0287-4547
- Publication type
Article
- DOI
10.4012/dmj.2023-012