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- Title
PREVALENCE, ANTIMICROBIAL RESISTANCE PROFILES OF CLINICAL AND SUBCLIN-ICAL MASTITIS IN LACTATING COW WITH ASSESSMENT OF TREATMENT TRIAL.
- Authors
Abo-Zaid, A. A.; El-Mekawey, M. F.; Soliman, A. H.; El-Neshwy, Wafaa M.
- Abstract
This study looked at the prevalence of bovine mastitis in a dairy farm in Egypt’s Ismalia Governorate, as well as the phenotypic and genotypic characteristics of the causative bacteria and their antimicrobial susceptibility. Also, a treatment trial with a combination of Cefalexin and Kanamycin was evaluated. The total prevalence of mastitis was 31.82% (119/374) at cow level and 17.01% (247/1452) at quarter level. 261 isolates were detected. As major microorganisms, 74.33% are Staphylococcus spp. and 25.67% are Streptococcus spp. The isolates were tested against 15 antimicrobial agents, with Gentamycin (CN), Ciprofloxacin (CIP), Cefalexin (CL), and Kanamycin (K) having the lowest percentage of resistant bacteria. Molecular characterization of isolated pathogens and antimicrobial resistance genes was performed by PCR on 15 isolates. blaTEM-1was the most frequently detected gene, followed by aadA1, dfrA1, cmlA, sul1, and tetA. 110 infected udder quarters were enrolled for 21 days to evaluate the treatment with Terrexine LC intramammary suspension 10g (Cefalexin ph. Eur 200mg, Kanamycin monosulfateph.Eur 100,000 I.U.) on six occasions at 12-hour intervals and Gentamycin intramuscular injection (1 cm/20 kg BW for 3-5 days) in cows with systemic reactions. A highly significant reduction was recorded for the log10 SSC, log10 TBC, and the level of LDH in milk after treatment compared to their level before treatment (P-value 0.0001***). The milking season, severity of mastitis, or type of microorganism isolated prior to treatment have no effect on the recovery rate (P-value 0.05). In conclusion, bacterial isolates in the present study revealed multidrug resistance to the majority of commonly used antimicrobial agents, so antibiotic usage must be restricted. PCR is a helpful technique for the detection of resistant bacteria. Treatment of bovine mastitis with a combination of antibiotics significantly improves the bacteriological cure, SSC, TBC, and the level of LDH in milk.
- Subjects
EGYPT; DRUG resistance in microorganisms; MASTITIS; BOVINE mastitis; INTRAMUSCULAR injections; COWS
- Publication
Slovenian Veterinary Research / Slovenski Veterinarski Zbornik, 2023, Vol 60, p327
- ISSN
1580-4003
- Publication type
Article
- DOI
10.26873/SVR-1618-2022