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- Title
IMPACT OF IL28B POLYMORPHISM ON TREATMENT INDUCED VIRAL CLEARANCE IN HCV INFECTED EGYPTIAN PATIENTS.
- Authors
Obada, Manar; El-Fert, Ashraf; Morad, Wesam; Ehsan, Nermin; Alhadad, Omkolsoum; El-Said, Hala
- Abstract
Background and objective: Interleukin (IL) 28B single nucleotide polymorphisms (SNP) was recently recognized as predictor of SVR in HCV infected patients treated by combination therapy of pegylatedinterferon (Peg-IFN) and ribavirin (RBV). The aim of the current study was to assess IL 28B polymorphism SNP (rs12979860) as a predictor of response to combined Peg-INF/RBV therapy in Egyptian chronic HCV infected patients. Design and methods: The study was conducted on 247 HCV infected patients and 100 apparently healthy control subjects. All patients were treated with PEG-IFN-α/ribavirin; and they were classified according to their response to treatment. Genotyping of IL28B rs12979860 was performed on peripheral blood DNA using polymerase chain reactionrestriction fragment length polymorphism (PCR-RFLP) assay. Results: The overall frequency of IL28B genotypes was 24.7%, 50.2% and 25.1% for genotypes CC, CT and TT respectively, while the response rate was 82%, 38.7% and 43.8% for genotypes CC, CT and TT respectively, moreover, genotype CC had increased probability to HCV clearance than both genotypes CT and TT with OR 7.71 (95%CI: 3.71-15.79). Conclusion: Genotyping of IL28B at SNP rs12979860 could be used as a guide to tailor treatment in Egyptian patients infected with HCV for better outcome.
- Subjects
INTERLEUKINS; HEPATITIS C treatment; SINGLE nucleotide polymorphisms; INTERFERONS; EGYPTIANS; POLYMERASE chain reaction; DISEASES
- Publication
Egyptian Journal of Biochemistry & Molecular Biology, 2014, Vol 32, Issue 2, p172
- ISSN
1687-1502
- Publication type
Article