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- Title
Use of bioreactors for culturing human retinal organoids improves photoreceptor yields.
- Authors
Ovando-Roche, Patrick; West, Emma L.; Branch, Matthew J.; Sampson, Robert D.; Fernando, Milan; Munro, Peter; Georgiadis, Anastasios; Rizzi, Matteo; Kloc, Magdalena; Naeem, Arifa; Ribeiro, Joana; Smith, Alexander J.; Gonzalez-Cordero, Anai; Ali, Robin R.
- Abstract
Background: The use of human pluripotent stem cell-derived retinal cells for cell therapy strategies and disease modelling relies on the ability to obtain healthy and organised retinal tissue in sufficient quantities. Generating such tissue is a lengthy process, often taking over 6 months of cell culture, and current approaches do not always generate large quantities of the major retinal cell types required. Methods: We adapted our previously described differentiation protocol to investigate the use of stirred-tank bioreactors. We used immunohistochemistry, flow cytometry and electron microscopy to characterise retinal organoids grown in standard and bioreactor culture conditions. Results: Our analysis revealed that the use of bioreactors results in improved laminar stratification as well as an increase in the yield of photoreceptor cells bearing cilia and nascent outer-segment-like structures. Conclusions: Bioreactors represent a promising platform for scaling up the manufacture of retinal cells for use in disease modelling, drug screening and cell transplantation studies.
- Subjects
ORGANOIDS; PLURIPOTENT stem cells; RETINAL rod photoreceptor cells; BIOREACTORS; CELL transplantation
- Publication
Stem Cell Research & Therapy, 2018, Vol 9, Issue 1, pN.PAG
- ISSN
1757-6512
- Publication type
Article
- DOI
10.1186/s13287-018-0907-0