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- Title
EFFECTS OF NEFERINE FROM NELUMBINIS PLUMULA ON OLEIC ACID-INDUCED HEPATIC STEATOSIS IN VITRO VIA ITS ANTIOXIDANT ACTIVITY.
- Authors
Yong Xie; Yi Zhang; Zebin Guo; HongIiang Zeng; Baodong Zheng
- Abstract
Hepatic steatosis, characterized by fa t accumulation in the liver, has led to the definition of series of disorders such as nonalcoholic fa tty liver disease (NAFLD), whose pathogenesis encompasses the deposition ofliverfree fa tty acids and associated factors such as oxidative stress. Our previous researches have demonstrated the protective effect of alkaloid compound neferine from Nelumbinis plumula against oxidative stress induced by t-BHP in hepatocyte, suggesting their involvement in the cytoprotection against oxidative stress. The aim of the present study was to investigate the antioxidant effect of neferine on oleic acid - induced hepatic steatosis. In this study, to evaluate protective effect of neferine on hepatic steatosis, we established experimental model by using a human hepatoblastoma cell line HepG2 cell, which was incubated with oleic acid. HepG2 cells were incubated with various concentrations of neferine or quercetin as positive control to evaluate their inhibitory effect on fa t - overloading and oxidative stress in volved in hepatic steatosis. Our results showed that 1 mM oleic acid could significantly stimulate hepatic steatosis, as evidenced by significant increase of total triglyceride secretion and lipid droplets accumulation in the cells. The protective effect of neferine against hepatic steatosis induced by oleic acid may be itssociated ivith the decreased level of reactive oxygen species (ROS) or thiobarbiluric acid-reactive substances (TBARS), and increased levels of glutathione (GSH) and antioxidant enzymes (superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx))in OA-induced HepG2 cells, suggesting cytoprotective of oxidative stress on their involvement in hepatic steatosis. Tire effects were comparable with the positive control quercetin. Overall, neferine can display a significant cytoprotective effect against hepatic steatosis via its antioxidant effect.
- Subjects
OLEIC acid; FATTY liver; ANTIOXIDANTS; OXIDATIVE stress; CYTOPROTECTION
- Publication
Current Topics in Nutraceutical Research, 2017, Vol 15, Issue 3/4, p195
- ISSN
1540-7535
- Publication type
Article