We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Aquaporins and Fetal Membranes From Diabetic Parturient Women: Expression Abnormalities and Regulation by Insulin.
- Authors
Bouvier, Damien; Rouzaire, Marion; Marceau, Geoffroy; Prat, Cécile; Pereira, Bruno; Lemarié, Romain; Deruelle, Philippe; Fajardy, Isabelle; Gallot, Denis; Blanchon, Loïc; Vambergue, Anne; Sapin, Vincent
- Abstract
<bold>Context: </bold>During pregnancy, aquaporins (AQPs) expressed in fetal membranes are essential for controlling the homeostasis of the amniotic volume, but their regulation by insulin was never explored in diabetic women.<bold>Objective: </bold>The aim of our study was to investigate the involvement of AQPs 1, 3, 8, and 9 expressed in fetal membranes in diabetic parturient women and the control of their expression by insulin.<bold>Design and Participants: </bold>From 129 fetal membranes in four populations (controls, type 1, type 2 [T2D], and gestational diabetes [GD]), we established an expression AQP profile. In a second step, the amnion was used to study the control of the expression and functions of AQPs 3 and 9 by insulin.<bold>Main Outcomes and Measures: </bold>The expression of transcripts and proteins of AQPs was studied by quantitative RT-PCR and ELISA. We analyzed the regulation by insulin of the expression of AQPs 3 and 9 in the amnion. A tritiated glycerol test enabled us to measure the impact of insulin on the functional characteristics. Using an inhibitor of phosphatidylinositol 3-kinase, we analyzed the insulin intracellular signaling pathway.<bold>Results: </bold>The expression of AQP3 protein was significantly weaker in groups T2D and GD. In nondiabetic fetal membranes, we showed for the amnion (but not for the chorion) a significant repression by insulin of the transcriptional expression of AQPs 3 and 9, which was blocked by a phosphatidylinositol 3-kinase inhibitor.<bold>Conclusion: </bold>In fetal membranes, the repression of AQP3 protein expression and functions observed in vivo is allowed by the hyperinsulinism described in pregnant women with T2D or GD.
- Subjects
AMNION; DIABETES; GESTATIONAL diabetes; FETAL membranes; INSULIN; MEMBRANE proteins
- Publication
Journal of Clinical Endocrinology & Metabolism, 2015, Vol 100, Issue 10, pE1270
- ISSN
0021-972X
- Publication type
journal article
- DOI
10.1210/jc.2015-2057