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- Title
Neuropilin-2 Expression in Papillary Thyroid Carcinoma: Correlation with VEGF-D Expression, Lymph Node Metastasis, and VEGF-D-Induced Aggressive Cancer Cell Phenotype.
- Authors
Hironao Yasuoka; Rieko Kodama; Mitsuyoshi Hirokawa; Yuuki Takamura; Akira Miyauchi; Michiya Inagaki; Tokio Sanke; Yasushi Nakamura
- Abstract
Context: Neuropilin-2 (Nrp2) is a coreceptor for vascular endothelial growth factor-D (VEGF-D) that is expressed on the surface of endothelial cells. Recently, Nrp2 was shown to play a role in lymph node metastasis and promotion of cancer cell migration. VEGF-D also promotes lymphangiogenesis, which in turn promotes tumor metastasis. Objective: The aim was to study the role of neuropilin-2 in lymph node metastasis in human papillary thyroid carcinoma (PTC). Design: Expression of Nrp2 was studied by immunohistochemistry and the relationship between Nrp2 expression and lymph node metastasis, VEGF-D expression and other established clinicopathological variables were analyzed in PTC. The effects of neutralizing anti-Nrp2 antibody on VEGF-D-induced invasion and migration were assessed in PTC cell lines. Results: Nrp2 expression was observed in 64.3% (36 of 56) of the PTC patients. Nrp2 expression was significantly correlated with lymph node metastasis (P = 0.0216) and VEGF-D expression (P = 0.0034). VEGF-D was shown to promote filopodia formation and cancer cell migration and invasion by K1 and B-CPAP cells. These responses were significantly blocked by neutralizing anti-Nrp2 antibody. Conclusion: Nrp2 expression was correlated with lymph node metastasis and VEGF-D expression in PTC. Our data also showed a role for Nrp2 in regulating VEGF-D-induced invasion and migration in vitro.
- Publication
Journal of Clinical Endocrinology & Metabolism, 2011, Vol 96, Issue 11, pE1857
- ISSN
0021-972X
- Publication type
Article
- DOI
10.1210/jc.2011-1180