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- Title
Development of a high yield expression and purification system for Domain I of Beta-2-glycoprotein I for the treatment of APS.
- Authors
McDonnell, Thomas; Pericleous, Charis; Laurine, Emmanuelle; Tommasi, Rita; Garza-Garcia, Acely; Giles, Ian; Ioannou, Yiannis; Rahman, Anisur
- Abstract
Background: In this paper we describe a novel method to achieve high yield bacterial expression of a small protein domain with considerable therapeutic potential; Domain I of Beta-2-glycoprotein I (β2GPI). β2GPI is intrinsic to the pathological progression of the Antiphospholipid Syndrome (APS). Patients develop autoantibodies targeting an epitope located on the N-terminal Domain I of β2GPI rendering this domain of interest as a possible therapeutic. Results: This new method of production of Domain I of β2GPI has increased the production yield by ~20 fold compared to previous methods in E.coli. This largely scalable, partially automated method produces 50-75 mg of pure, folded, active Domain I of β2GPI per litre of expression media. Conclusion: The application of this method may enable production of Domain I on sufficient scale to allow its use as a therapeutic.
- Subjects
PROTEIN expression; GLYCOPROTEIN genetics; DISEASE progression; ANTIPHOSPHOLIPID syndrome; AUTOANTIBODIES; N-terminal residues; GENETICS
- Publication
BMC Biotechnology, 2015, Vol 15, p1
- ISSN
1472-6750
- Publication type
Article
- DOI
10.1186/s12896-015-0222-0