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- Title
Statistical Optimization of Reverse Phase High Performance Liquid Chromatography for the Analysis of Caffeine Paracetamol and its Degradation Product p-aminophenol.
- Authors
Crevar, Milkica; Ivkovic, Branka; Vladimirov, Sote; Kuntic, Vesna; Vujic, Zorica
- Abstract
Paracetamol, analgoantipyretic and anti-inflammatory drug, is available in numerous pharmaceutical formulations in common combination with caffeine and some other drug substances. p-aminophenol is paracatamol's process-related impurity that also may be present in formulations containing paracetamol. This paper presents a RP-HPLC method for simultaneous determination of caffeine, paracetamol and p-aminophenol, using chromatographic system Hewlett Packard 1100 series. In defining optimal RP-HPLC chromatographic conditions for the separation of these three compounds, experimental design was applied. Completely separation was achieved using Zorbax Extend C18 column (150 mm × 4.6 mm, 5 μm), with mobile phase consisting of methanol-phosphate buffer pH 6 (20:80 v/v), flow rate of 1 ml/min, and column temperature of 30 °C. UV detection was performed at 230 nm.
- Subjects
ACETAMINOPHEN; ANTI-inflammatory agents; CAFFEINE; CHROMATOGRAPHIC detectors; EXPERIMENTAL design
- Publication
Acta Chimica Slovenica, 2008, Vol 55, Issue 3, p665
- ISSN
1318-0207
- Publication type
Article