We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Genetic diversity of astroviruses detected in wild aquatic birds in Hong Kong.
- Authors
Ng, Daisy Y. M.; Sun, Wanying; Sit, Thomas H. C.; Brackman, Christopher J.; Tse, Anne C. N.; Bui, Christine H. T.; Tang, Amy W. Y.; Wong, Andrew N. C.; Tsang, Andrew T. L.; Koo, Joe C. T.; Cheng, Samuel M. S.; Peiris, Malik; Chin, Alex W. H.; Poon, Leo L. M.
- Abstract
Wild waterfowl serve as a reservoir of some astroviruses. Fecal samples from wild waterfowl collected at Hong Kong's Marshes were tested using pan-astrovirus reverse transcription-PCR. Positive samples underwent subsequent host identification using DNA barcoding. Based on deduced partial sequences, noteworthy samples from three astrovirus groups (mammalian, avian and unclassified astroviruses) were further analyzed by next-generation sequencing. One sample of Avastrovirus 4 clade, MP22-196, had a nearly complete genome identified. The results of ORF2 phylogenetic analysis and genetic distance analysis indicate that Avastrovirus 4 is classified as a distinct subclade within Avastrovirus. MP22-196 has typical astrovirus genome characteristics. The unique characteristics and potential differences of this genome, compared to other avian astrovirus sequences, involve the identification of a modified sgRNA sequence situated near the ORF2 start codon, which precedes the ORF1b stop codon. Additionally, the 3' UTR of MP22-196 is shorter than other avian astroviruses. This study expands our understanding of the Avastrovirus 4 clade.
- Subjects
HONG Kong (China); WATER birds; ASTROVIRUSES; GENETIC variation; GENETIC barcoding; GENETIC distance
- Publication
Virology Journal, 2024, Vol 21, Issue 1, p1
- ISSN
1743-422X
- Publication type
Article
- DOI
10.1186/s12985-024-02423-w