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- Title
Cloning and Expression Pattern of a Gene Encoding a Putative Plastidic ATP/ADP Transporter from Helianthus tuberosus L.
- Authors
Kun Meng; Tuan-Jie Chang; Xiang Liu; Song-Biao Chen; Yong-Qin Wang; Ai-Jun Sun; Hong-Lin Xu; Xiao-Li Wei; Zhen Zhu
- Abstract
Herein, we report the cloning and molecular characterization of a full cDNA encoding a putative plastidic ATP/ADP transporter, designated HtAATP, for Helianthus tuberosus L. The ATP/ADP translocator protein was isolated from the tuber-cDNA library of H. tuberosus for the first time. The predicted HtAATP protein was judged as a plastidic ATP/ADP translocator protein from its high homology at the amino acid sequence level to the two Arabidopsis thaliana plastidic ATP/ADP translocator proteins AATP1 and AATP2 (84.8% and 79.9% identity, respectively). Amino acid sequence analysis of the primary structure of HtAATP revealed that it belonged to the plastidic ATP/ADP transporter family. Hydropathy prediction indicated that HtAATP gene product is a highly hydrophobic membrane protein that contains 10 transmembrane domains to form a spanning topology. Southern blotting analysis showed that the HtAATP gene is a single-copy gene in the H. tuberosus genome. Tissue distribution analysis showed that the HtAATP gene is prominently expressed in sink tissues. A stable expression pattern in tubers at different developmental stages implies an active involvement of HtAATP during carbohydrate formation. (Managing editor: Li-Hui ZHAO)
- Subjects
AMINO acids; PROTEINS; CLONING; ASTERACEAE; PLANT genetics; GENETICS
- Publication
Journal of Integrative Plant Biology, 2005, Vol 47, Issue 9, p1123
- ISSN
1672-9072
- Publication type
Article
- DOI
10.1111/j.1744-7909.2005.00119.x