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- Title
Fibroblast growth factor 21 enhances cholesterol efflux in THP-1 macrophage-derived foam cells.
- Authors
WEI SHANG; XUEJING YU; HONGLIAN WANG; TIELIN CHEN; YING FANG; XIANGGUI YANG; PUHUI ZHOU; FANG NIE; QIN ZHOU; JIANZHONG ZHOU
- Abstract
Fibroblast growth factor 21 (FGF21) is a novel metabolic regulator. The present study aimed to investigate the effect of FGF21 on cholesterol efflux and the expression of ATP binding cassette (ABC) A1 and G1 in human THP-1 macrophage-derived foam cells. Furthermore, the present study aimed to investigate the role of the liver X receptor (LXR) α in this process. A model of oxidized low-density lipoprotein-induced foam cells from human THP-1 cells was established. The effect of FGF21 on cholesterol efflux was analyzed using a liquid scintillation counter. The expression of ABCA1 and ABCG1 was determined using quantitative polymerase chain reaction and western blot analyses. FGF21 was found to enhance apolipoprotein A1- and high-density lipoprotein-mediated cholesterol efflux. FGF21 was also observed to increase the mRNA and protein expression of ABCA1 and ABCG1. Furthermore, LXRα-short interfering RNA attenuated the stimulatory effects induced by FGF21. These findings suggest that FGF21 may have a protective effect against atherosclerosis by enhancing cholesterol efflux through the induction of LXRα-dependent ABCA1 and ABCG1 expression.
- Subjects
FIBROBLAST growth factor 2; MACROPHAGES; LIQUID scintillation counting; POLYMERASE chain reaction; APOLIPOPROTEIN A; ATHEROSCLEROSIS; LOW density lipoproteins
- Publication
Molecular Medicine Reports, 2015, Vol 11, Issue 1, p503
- ISSN
1791-2997
- Publication type
Article
- DOI
10.3892/mmr.2014.2731