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- Title
Lipidomic Characterization of Oocytes at Single-Cell Level Using Nanoflow Chromatography-Trapped Ion Mobility Spectrometry-Mass Spectrometry.
- Authors
Zhu, Pujia; Bu, Guowei; Hu, Ruifeng; Ruan, Xianqin; Fu, Rongrong; Zhang, Zhourui; Wan, Qiongqiong; Liu, Xin; Miao, Yiliang; Chen, Suming
- Abstract
Mass spectrometry (MS)-based lipidomic has become a powerful tool for studying lipids in biological systems. However, lipidome analysis at the single-cell level remains a challenge. Here, we report a highly sensitive lipidomic workflow based on nanoflow liquid chromatography and trapped ion mobility spectrometry (TIMS)-MS. This approach enables the high-coverage identification of lipidome landscape at the single-oocyte level. By using the proposed method, comprehensive lipid changes in porcine oocytes during their maturation were revealed. The results provide valuable insights into the structural changes of lipid molecules during porcine oocyte maturation, highlighting the significance of sphingolipids and glycerophospholipids. This study offers a new approach to the single-cell lipidomic.
- Subjects
ION mobility spectroscopy; OVUM; MASS spectrometry; LIQUID chromatography; BIOLOGICAL systems; ION traps
- Publication
Molecules, 2023, Vol 28, Issue 10, p4202
- ISSN
1420-3049
- Publication type
Article
- DOI
10.3390/molecules28104202