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- Title
Effects of Cold Storage in Krebs-Henseleit Solution at +4 °C on Vasoreactivity of the Rat Thoracic Aorta .
- Authors
DEMİREL, Sadettin
- Abstract
Objective: This study aimed to investigate whether endothelium-dependent and -independent relaxation responses can be preserved intact in the rat thoracic aorta after storage for 3, 6, and 18 hours in Krebs-Henseleit solution at +4 °C. Materials and Methods: Isolated organ bath model and 10-12 weeks old male Wistar rats were used to perform the experiments. To investigate the effect of endothelium-dependent relaxation factors, the cyclooxygenase inhibitor indomethacin was added to the KrebsHenseleit solution to inhibit endogenous prostanoid synthesis. Submaximal contraction response was obtained with a single dose of phenylephrine and then acetylcholine was administered cumulatively (10-9 -10-4 M) to induce endothelium-dependent relaxation responses. Besides, smooth muscle-dependent relaxation responses were obtained by applying sodium nitroprusside cumulatively (10-9 -10-5 M) following precontraction induced by phenylephrine. The statistical significance level was considered as p<0.05. Results: Thoracic aorta segments (0 hours, control) exhibited a mean contraction response of 3123 mg against 10-5 M phenylephrine. Although the responses slightly reduced in vascular rings stored for 3, 6, and 18 hours, no significant difference was observed (p>0.05). Besides, cumulatively administered acetylcholine did not cause a significant change in endotheliumdependent relaxation responses (p>0.05). Similarly, sodium nitroprusside did not modulate the endothelium-independent relaxation responses in aortic segments after storage for 3, 6, or 18 hours (p>0.05). Conclusion: In the present study, the first physiological findings have been obtained that the endothelium-dependent and -independent contraction-relaxation responses of rat thoracic aortas can be preserved intact after storage periods of 3, 6, or 18 hours in Krebs-Henseleit solution at +4°C.
- Subjects
STATISTICS; ENDOTHELIUM; MUSCLE contraction; ANIMAL experimentation; PHENYLEPHRINE; ONE-way analysis of variance; THORACIC aorta; VASODILATION; RELAXATION for health; RATS; T-test (Statistics); DESCRIPTIVE statistics; DATA analysis software; DATA analysis; PHYSIOLOGIC salines; CRYOPRESERVATION of organs, tissues, etc.
- Publication
Balikesir Health Sciences Journal / Balıkesir Sağlık Bilimleri Dergisi, 2022, Vol 11, Issue 3, p456
- ISSN
2146-9601
- Publication type
Article
- DOI
10.53424/balikesirsbd.1141819