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- Title
An economically and environmentally acceptable synthesis of chiral drug intermediate L-pipecolic acid from biomass-derived lysine via artificially engineered microbes.
- Authors
Cheng, Jie; Huang, Yuding; Mi, Le; Chen, Wujiu; Wang, Dan; Wang, Qinhong
- Abstract
Deficiency in petroleum resources and increasing environmental concerns have pushed a bio-based economy to be built, employing a highly reproducible, metal contaminant free, sustainable and green biomanufacturing method. Here, a chiral drug intermediate L-pipecolic acid has been synthesized from biomass-derived lysine. This artificial bioconversion system involves the coexpression of four functional genes, which encode L-lysine α-oxidase from Scomber japonicus, glucose dehydrogenase from Bacillus subtilis, Δ1-piperideine-2-carboxylase reductase from Pseudomonas putida, and lysine permease from Escherichia coli. Besides, a lysine degradation enzyme has been knocked out to strengthen the process in this microbe. The overexpression of LysP improved the L-pipecolic acid titer about 1.6-folds compared to the control. This engineered microbial factory showed the highest L-pipecolic acid production of 46.7 g/L reported to date and a higher productivity of 2.41 g/L h and a yield of 0.89 g/g. This biotechnological L-pipecolic acid production is a simple, economic, and green technology to replace the presently used chemical synthesis.
- Subjects
CHIRAL drugs synthesis; PIPECOLIC acid; LYSINE; BIOCONVERSION; MICROORGANISMS
- Publication
Journal of Industrial Microbiology & Biotechnology, 2018, Vol 45, Issue 6, p405
- ISSN
1367-5435
- Publication type
Article
- DOI
10.1007/s10295-018-2044-2