We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Identifying the effect of vancomycin on health care–associated methicillin-resistant Staphylococcus aureus strains using bacteriological and physiological media.
- Authors
Rajput, Akanksha; Poudel, Saugat; Tsunemoto, Hannah; Meehan, Michael; Szubin, Richard; Olson, Connor A; Seif, Yara; Lamsa, Anne; Dillon, Nicholas; Vrbanac, Alison; Sugie, Joseph; Dahesh, Samira; Monk, Jonathan M; Dorrestein, Pieter C; Knight, Rob; Pogliano, Joe; Nizet, Victor; Feist, Adam M; Palsson, Bernhard O
- Abstract
Background The evolving antibiotic-resistant behavior of health care–associated methicillin-resistant Staphylococcus aureus (HA-MRSA) USA100 strains are of major concern. They are resistant to a broad class of antibiotics such as macrolides, aminoglycosides, fluoroquinolones, and many more. Findings The selection of appropriate antibiotic susceptibility examination media is very important. Thus, we use bacteriological (cation-adjusted Mueller-Hinton broth) as well as physiological (R10LB) media to determine the effect of vancomycin on USA100 strains. The study includes the profiling behavior of HA-MRSA USA100 D592 and D712 strains in the presence of vancomycin through various high-throughput assays. The US100 D592 and D712 strains were characterized at sub-inhibitory concentrations through growth curves, RNA sequencing, bacterial cytological profiling, and exo-metabolomics high throughput experiments. Conclusions The study reveals the vancomycin resistance behavior of HA-MRSA USA100 strains in dual media conditions using wide-ranging experiments.
- Subjects
METHICILLIN-resistant staphylococcus aureus; METHICILLIN; VANCOMYCIN; VANCOMYCIN resistance; MICROBIAL exopolysaccharides; HEALTH behavior; RNA sequencing
- Publication
GigaScience, 2021, Vol 10, Issue 1, p1
- ISSN
2047-217X
- Publication type
Article
- DOI
10.1093/gigascience/giaa156