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- Title
Immunogenicity of the extracellular copper/zinc superoxide dismutase of the filarial parasite Acanthocheilonema viteae delivered by a two-phase vaccine strain ofSalmonella typhimurium.
- Authors
LATTEMANN, CLAUS T.; YAN, ZHENG-XIN; MATZEN, ARNE; MEYER, THOMAS F.; APFEL, HEIKO
- Abstract
The recombinant extracellular copper/zinc superoxide dismutase of the filarial parasite Acanthocheilonema viteae (AVSOD2) was cloned in an expression vector under control of the bacteriophage T7 promoter and the resulting plasmid pLAT7 was introduced in tha aroA attenuated Salmonella typhimurium vaccine strain SL3261::pYZ84. This vaccine strain carries a chromosomally integrated two phase expression system containing inducible T7 RNA polymerase. The recombinant AVSOD2 was efficiently expressed, constituting up to 5% of the total bacterial protein. Furthermore, the plasmid vector containing the AVSOD2 cDNA was shown to be stable over a long period of time in the vaccine strain without antibiotic selection in vitro and in vivo. Jirds which were immunised orally with the recombinant vaccine strain expressing the A. viteae EC-SOD produced a strong humoral immune response..
- Subjects
SUPEROXIDE dismutase; SALMONELLA typhimurium; FILARIASIS
- Publication
Parasite Immunology, 1999, Vol 21, Issue 4, p219
- ISSN
0141-9838
- Publication type
Article
- DOI
10.1046/j.1365-3024.1999.00207.x