We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Corticosterone Actions on the Hippocampal Brain-Derived Neurotrophic Factor Expression are Mediated by Exon IV Promoter.
- Authors
Hansson, A. C.; Sommer, W. H.; Metsis, M.; Strömberg, I.; Agnati, L. F.; Fuxe, K.
- Abstract
Brain-derived neurotrophic factor (BDNF) expression is strongly regulated by adrenocorticosteroids via activated gluco- and mineralocorticoid receptors. Four separate promoters are located upstream of the BDNF noncoding exons I to IV and may thus be involved in adrenocorticosteroid-mediated gene regulation. In adrenalectomised rats, corticosterone (10 mg/kg s.c.) induces a robust down-regulation of both BDNF mRNA and protein levels in the hippocampus peaking at 2–8 h. To study the role of the individual promoters in the corticosterone response, we employed exon-specific riboprobe in situ hybridisation as well as real-time polymerase chain reaction (PCR) in the dentate gyrus. We found a down-regulation, mainly of exon IV and the protein-coding exon V, in nearby all hippocampal subregions, but exon II was only down-regulated in the dentate gyrus. Exon I and exon III transcripts were not affected by corticosterone treatment. The results could be confirmed with real-time PCR in the dentate gyrus. It appears as if the exon IV promoter is the major target for corticosterone-mediated transcriptional regulation of BDNF in the hippocampus.
- Subjects
CORTICOSTERONE; MINERALOCORTICOIDS; GLUCOCORTICOIDS; GENE expression; GENETIC regulation; EXONS (Genetics); GENETICS
- Publication
Journal of Neuroendocrinology, 2006, Vol 18, Issue 2, p104
- ISSN
0953-8194
- Publication type
Article
- DOI
10.1111/j.1365-2826.2005.01390.x