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- Title
The Hypothalamic-Pituitary-Adrenal Axis Response to Endotoxin is Attenuated During Lactation.
- Authors
Shanks; Windle; Perks; Wood; Ingram; Lightman; Shanks, Nola
- Abstract
Pregnancy and lactation are times of prolonged physiological changes affecting the neuroendocrine and immunological systems. One well-characterized change is the neuroendocrine hyporesponsiveness to acute stressful stimuli. We have now designed studies to see whether there is an alteration in the response of the hypothalamic-pituitary-adrenal (HPA) axis to an immunological inflammatory challenge and to ascertain whether lactating animals show altered neural and endocrine responses to inflammatory stimuli. Lactating (day 9–12 postpartum) or virgin control Sprague-Dawley female rats were injected with either 200 μg of endotoxin (lipopolysaccharide, LPS ) or sterile saline given i.p. Trunk blood or jugular blood was collected from the animals at 2 h or hourly over 6 h after injection. Both plasma adrenocorticotropic hormone (ACTH) and corticosterone concentrations were significantly higher in saline treated lactating animals compared with the virgin group. LPS significantly elevated circulating levels of plasma ACTH and corticosterone in both virgin and lactating animals compared with saline controls, however, hormone responses to LPS were significantly reduced in lactating animals relative to virgin controls. Corticosterone-binding globulin concentrations were lower in lactating animals compared to virgin animals and LPS decreased concentrations in virgin, but not lactating rats. Analysis of cfos mRNA in the paraventricular nucleus (PVN) of the hypothalamus revealed that 2 h following injection there was a increase in cfos expression only in the virgin animals treated with LPS, compared to all other treatment conditions. Corticotropin-releasing hormone (CRH) mRNA expression was overall greater in virgin animals, but was increased to similar extent in both virgin and lactating animals treated with LPS. Primary arginine vasopressin (AVP) mRNA transcripts were increased 2 h following LPS injection, but a greater increase in expression was seen in virgin...
- Subjects
ENDOTOXINS; LACTATION; REPRODUCTION endocrinology; GLUCOCORTICOIDS; PHYSIOLOGY
- Publication
Journal of Neuroendocrinology, 1999, Vol 11, Issue 11, p857
- ISSN
0953-8194
- Publication type
Article
- DOI
10.1046/j.1365-2826.1999.00400.x