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- Title
NGL family PSD-95–interacting adhesion molecules regulate excitatory synapse formation.
- Authors
Seho Kim; Burette, Alain; Hye Sun Chung; Seok-Kyu Kwon; Jooyeon Woo; Hyun Woo Lee; Karam Kim; Hyun Kim; Weinberg, Richard J.; Eunjoon Kim
- Abstract
Synaptic cell adhesion molecules (CAMs) regulate synapse formation through their trans-synaptic and heterophilic adhesion. Here we show that postsynaptic netrin-G ligand (NGL) CAMs associate with netrin-G CAMs in an isoform-specific manner and, through their cytosolic tail, with the abundant postsynaptic scaffold postsynaptic density-95 (PSD-95). Overexpression of NGL-2 in cultured rat neurons increased the number of PSD-95-positive dendritic protrusions. NGL-2 located on heterologous cells or beads induced functional presynaptic differentiation in contacting neurites. Direct aggregation of NGL-2 on the surface membrane of dendrites induced the clustering of excitatory postsynaptic proteins. Competitive inhibition by soluble NGL-2 reduced the number of excitatory synapses. NGL-2 knockdown reduced excitatory, but not inhibitory, synapse numbers and currents. These results suggest that NGL regulates the formation of excitatory synapses.
- Subjects
CELL adhesion molecules; SYNAPSES; LIGANDS (Biochemistry); NEURONS; EXCITATORY amino acids; NEURAL transmission; RATS
- Publication
Nature Neuroscience, 2006, Vol 9, Issue 10, p1294
- ISSN
1097-6256
- Publication type
Article
- DOI
10.1038/nn1763