We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Effects of sevoflurane on metalloproteinase and natural killer group 2, member D (NKG2D) ligand expression and natural killer cellmediated cytotoxicity in breast cancer: an in vitro study.
- Authors
Hyae Jin Kim; Soeun Jeon; Hyeon Jeong Lee; Jaeho Bae; Hyun-Su Ri; Jeong-Min Hong; Sung In Paek; Seul Ki Kwon; Jae-Rin Kim; Seungbin Park; Eun-Jung Yun
- Abstract
Background: We investigated the effects of sevoflurane exposure on the expression of matrix metalloproteinase (MMP), expression and ablation of natural killer group 2, member D (NKG2D) ligands (UL16-binding proteins 1-3 and major histocompatibility complex class I chain-related molecules A/B), and natural killer (NK) cell-mediated cytotoxicity in breast cancer cells. Methods: Three human breast cancer cell lines (MCF-7, MDA-MB-453, and HCC-70) were incubated with 0 (control), 600 (S6), or 1200(1M (S12) sevoflurane for 4 h. The gene expression of NKG2D ligands and their protein expression on cancer cell surfaces were measured using multiplex polymerase chain reaction (PCR) and flow cytometry, respectively. Protein expression of MMP-1 and -2 and the concentration of soluble NKG2D ligands were analyzed using western blotting and enzyme-linked immunosorbent assays, respectively. Results: Sevoflurane downregulated the mRNA and protein expression of the NKG2D ligand in a dose-dependent manner in MCF-7, MDA-MB-453, and HCC-70 cells but did not affect the expression of MMP-1 or -2 or the concentration of soluble NKG2D ligands in the MCF-7, MDA-MB-453, and HCC-70 cells. Sevoflurane attenuated NK cell-mediated cancer cell lysis in a dose-dependent manner in MCF-7, MDA-MB-453, and HCC-70 cells (P = 0.040, P = 0.040, and P = 0.040, respectively). Conclusions: Our results demonstrate that sevoflurane exposure attenuates NK cell-mediated cytotoxicity in breast cancer cells in a dose-dependent manner. This could be attributed to a sevoflurane-induced decrease in the transcription of NKG2D ligands rather than sevoflurane-induced changes in MMP expression and their proteolytic activity.
- Subjects
GENE expression; CYTOTOXINS; BREAST cancer; SEVOFLURANE; CELL-mediated cytotoxicity
- Publication
Korean Journal of Anesthesiology, 2023, Vol 76, Issue 6, p627
- ISSN
2005-6419
- Publication type
Article
- DOI
10.4097/kja.23323