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- Title
Sildenafil prevents renal dysfunction in contrast media-induced nephropathy in Wistar rats.
- Authors
Almeida, Lais Salles de; Barboza, Jamila Rodrigues; Freitas, Flávia Priscila Santos; Porto, Marcella Leite; Vasquez, Elisardo Corral; Meyrelles, Silvana Santos; Gava, Agata Lages; Pereira, Thiago Melo Costa
- Abstract
Contrast-induced nephropathy (CIN) is an iatrogenic medical event in stable cardiology patients that may lead to acute renal failure. There is no current successful therapy to manage CIN. Increasing evidence in experimental models and humans has suggested that this disease is associated with renal tubular and vascular injury triggered by oxidative stress. Considering the importance of reactive oxygen species (ROS) generation in the pathogenesis of CIN, the goal of the present study was to evaluate the effects of sildenafil on CIN development. Male Wistar rats were divided into control, CIN, and CIN pretreated with sildenafil (50 mg/kg/day). CIN was induced by water deprivation, NG-nitro-L-arginine methyl ester + indomethacin injections (10 mg/kg, intraperitoneally) and intravenous iohexol administration (3 g/kg). Renal function was evaluated through glomerular filtration rate (GFR), renal blood flow (RBF), plasma creatinine, uremia, and proteinuria. Oxidative stress was assessed by flow cytometry for intracellular ROS. Treatment with sildenafil attenuated the marked reduction of GFR and RBF in the CIN group. Moreover, sildenafil treatment in CIN rats reduced plasma creatinine, uremia, and proteinuria. Flow cytometry demonstrated that sildenafil attenuated the ROS production in the CIN group. These data suggest that sildenafil may be a new therapeutic agent to prevent CIN through its ability to preserve renal function and attenuate oxidative stress.
- Subjects
KIDNEY disease treatments; SILDENAFIL; LABORATORY rats; OXIDATIVE stress; ACUTE kidney failure; THERAPEUTICS
- Publication
Human & Experimental Toxicology, 2016, Vol 35, Issue 11, p1194
- ISSN
0960-3271
- Publication type
Article
- DOI
10.1177/0960327115626582