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- Title
Multiplex Portable Biosensor for Bacteria Detection.
- Authors
Kaci, Karim; Enebral-Romero, Estefanía; Martínez-Periñán, Emiliano; Garrido, Marina; Pérez, Emilio M.; López-Diego, David; Luna, Mónica; González-de-Rivera, Guillermo; García-Mendiola, Tania; Lorenzo, Encarnación
- Abstract
An advanced, cost-effective, and portable DNA biosensor capable of detecting multiple bacteria simultaneously has been developed. The biosensor comprises a fast and inexpensive potentiostat that controls the applied potential to a screen-printed electrochemical array platform functionalized with MoS2 flakes and bacterial DNA probes. The current response obtained by à la carte thionine functionalized carbon nanodots (Ty-CDs) is monitored as an electrochemical indicator of the hybridization event. The design of the potentiostat prioritizes achieving an optimal signal-to-noise ratio and incorporates a user-friendly interface compatible with various devices, including computers, mobile phones, and tablets. The device is compact, lightweight, and manufactured at a low cost. The key components of the potentiostat include a data acquisition board capable of analyzing multiple samples simultaneously and a controller board. The results of this study confirm the ability of the multiplex portable biosensor to successfully detect specific bacterial DNA sequences, demonstrating its reliability and superior performance compared with a traditional, more complex, and laboratory-oriented potentiostat.
- Subjects
BIOSENSORS; BACTERIAL DNA; DNA probes; SIGNAL-to-noise ratio
- Publication
Biosensors (2079-6374), 2023, Vol 13, Issue 11, p958
- ISSN
2079-6374
- Publication type
Article
- DOI
10.3390/bios13110958