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- Title
The Establishment of a Cell Strain (PC-1) from a Human Lung Cancer.
- Authors
HAYATA, YOSHIHIRO; TSUJI, KEIJIRO; OHO, KENKICHI; KOH, TETSUKICHI; SHIMOSATO, YUKIO
- Abstract
A human lung cancer cell strain (PC-1) was established in vitro and maintained in continuous growth for more than two and a half years. The material used for explanation was a metastatic lymph node of a case of poorly differentiated epidermoid carcinoma consisting predominantly of small anaplastic cells of a polygonal cell type. The cultured cell grew attached to a glass surface in layered heaps and were epidermoid ultrastructurally.Chromosomal analysis of the cells at the 7th passage culture revealed that all mitotic cells with the chromosome number of 46 or more exhibited the karyotypic abnormality of Dp+. Tumors developed after hetcrotransplantation of the cultured cells into the hamster's cheek pouch showed features of epidermoid carcinoma with keratinization indicating neoplastic cell origin of the strain. Doubling time of the total cell population was 72 hours.Histogenesis of the small cell anaplastic carcinoma was discussed.
- Publication
Japanese Journal of Clinical Oncology, 1974, Vol 4, Issue 2, p115
- ISSN
0368-2811
- Publication type
Article