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- Title
IN VITRO REGENERATION FROM APICAL BUD EXPLANT OF ROSMARINUS OFFICINALIS L.-AN IMPORTANT MEDICINAL PLANT.
- Authors
D., LEELAVATHI; KUPPAN, Narendra
- Abstract
Apical bud explant measuring 1.0 cm was excised from in vitro plant of Rosmarinus officinalis and cultured on MSBM fortified with cytokinin BAP (8.88µ?) and auxins in different concentrations of IAA (2.85 µ?, 5.70 µ?, 8.5 5 µ?, 11.40 µ?, 14.25 µ?) and 2,4-D (2.26 µ?, 4.52 µ?, 6.78 µ?, 9.04 µ?, 11.30 µ?) respectively in order to induce callus MSBM + BAP (8.88 µ?) + IAA (5.70 µ?) was found to be the best medium for the induction and growth of whitish green compact callus from the apical bud explants of Rosmarinus officinalis when compared to the callus derived from MSBM + BAP (8.88 µ?) + 2,4-D (4.52 µ?). It was observed in the present study that MSBM and BAP (8.88 µ?) with IAA (2.85 µ?) was the most suitable medium for initiation shoots and multiple shoot formation from in vitro apical bud of R. officinalis. These axenic plants were hardened and successfully established in the field with 80% survival frequency.
- Subjects
SHOOT apical meristems; ROSEMARY; PLANT growing media
- Publication
Banat's Journal of Biotechnology, 2013, Vol 4, Issue 8, p14
- ISSN
2068-4673
- Publication type
Article
- DOI
10.7904/2068-4738-iv(08)-14