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- Title
Preparo da semente de arroz para execução do teste de tetrazólio.
- Authors
Leitzke Carvalho, Ireni; Eduardo Meneghello, Géri; Madruga Tunes, Lilian; Jácome Costa, Caroline; Nogueira Soares, Vanessa
- Abstract
The calibration of methodologies able to identifying the quality of a rice seed lot, quickly and efficiently through viability tests such as tetrazolium, still lacks adjustments. The aim of this study was to evaluate seed preparation and cutting alternatives to assess the viability of rice seeds. The study was conducted in two stages: a) need to remove the bark (palea and lemma) and b) cutting the seeds to evaluate the viability of rice seeds. Seeds of four cultivars of irrigated rice (Puitá Inta CL, BR IRGA 424, SCS 116 Satoru and SCS 117 CL) and two cultivars of dry rice (BRS Serra Dourada and BRS Esmeralda) were used, each represented by two lots, totaling twelve lots evaluated. The seed lots were initial characterized in terms of water content, germination test, and seedling's emergence. Subsequently, the study was divided in two stages, being conducted in a sequential manner, the result of stage I being used as a basis to proceed in stage II. In the first step, it was evaluated the need of the removal or not the palea and lemma for the execution of the tetrazolium test. In the second step, we evaluated the seeds without the palea and lemma for the longitudinal, transverse cut or uncut. The results indicated that for the evaluation of the viability of rice seeds by the tetrazolium test it is recommended the removal of the palea and lemma, being more efficient the longitudinal cut in the distal half of the embryonic axis. Removal of the palea and lemma associated with the longitudinal cut in the distal half of the embryo facilitate the evaluation of the viability of rice seeds by the tetrazolium test.
- Subjects
RICE seeds; SEED quality; TETRAZOLIUM; RICE; SEED viability; PLANT cuttings
- Publication
Colloquium Agrariae, 2019, Vol 15, Issue 1, p51
- ISSN
1809-8215
- Publication type
Article
- DOI
10.5747/ca.2019.v15.n1.a270