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- Title
Investigation of effects of walnut leaf water extract on cortex neuron and LN405 cancer line: in vitro assay.
- Authors
Yeşilyurt, Fatma; Taghizadehghalehjoughi, Ali; Yılmaz, Ayşegül; Karakaya, Songül; Hacımüftüoğlu, Ahmet
- Abstract
Objective: The walnut tree leaves and seeds are used in medicine and cosmetics industry, cores and shells. The aim of my study was to investigate the effects of glutamate toxicity model on cortex neuron and LN405 cell cultures using different doses of walnut leaf water extract (CYSE). Methods: Neuron cultures, was obtained from the department of medical Pharmacology, Ataturk University, Erzurum, Turkey. Walnut leaves were softened with distilled water for 8 hours/3 days at 30-35 °C. The aqueous extract was filtered, freeze-dried. CYSE was used at doses of ', 25, 50, 75, '00 and '25 micrograms (pg) and glutamate at ' 0 -5 mM. Eight experimental groups were administered to neuron and LN405 cells. The cytotoxicity was examined after 24 hours of treatment. MTT results were analyzed by one way ANOVA method in SPSS, IBM 2'.00 program. Results: The vitality ratio of the control groups was defined as '00%. Glutamate in cortex neurons showed a minimum viability rate of ' 0 -5 mM 62%. In CYSE, the highest viability rate of CYSE was found to be 25 pgr. In LN405 cancer cells, glutamate has a vitality rate of ' 0 -5 mM 97%. The highest mortality rate was 67%, and CYSE was 25 pgr and there was a significant difference between the control group. Conclusion: Pereira et al. (2007) investigated the antioxidant activities of leaf extracts of walnut leaf and found strong antioxidant capacity. The antioxidant activity in our study increased the viability of neurons while cancer cells were killed.
- Subjects
TURKEY; PHARMACOLOGY; NEURONS; WATER softening; DISTILLED water; OXIDANT status; ENTORHINAL cortex
- Publication
Anatomy: International Journal of Experimental & Clinical Anatomy, 2019, Vol 13, Issue S1, pS66
- ISSN
1307-8798
- Publication type
Article