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- Title
Survivin Impairs the Apoptotic Machinery in CD4<sup>+</sup> T Cells of Patients with Ulcerative Colitis.
- Authors
Feng, Bai-Sui; Ma, Na; Zhang, Yuan-Yi; Gao, Han; Zhang, Cui; Li, Gengfeng; Liu, Zhanju; Feng, Yisheng; Yu, Hai-Qiong; Xiao, Liang; Liu, Zhi-Gang; Yang, Ping-Chang
- Abstract
Background: The increase in CD4+ T cell infiltration and overproduction of CD4+ T cell-associated cytokines have been observed in the inflamed colon mucosa of patients with ulcerative colitis (UC); the underlying mechanisms are not fully understood. Survivin plays a critical role in the interference with apoptotic machinery. This study aims to elucidate the role of survivin in the interference with the apoptotic machinery in CD4+ T cells of UC patients. Methods: Peripheral blood samples were collected from UC patients (UC group) and healthy subjects (healthy group). The apoptotic status in CD4+ T cells was analyzed by flow cytometry. Results: We observed that the expression of survivin was significantly higher in CD4+ T cells of UC patients than in healthy subjects. UC CD4+ T cells were resistant to apoptosis induction. A complex of survivin and c-Myc, the transcription factor of FasL, was detected in CD4+ T cells in UC patients, which prevented the binding of c-Myc to the FasL promoter and interfered with the expression of FasL. Increased expression of survivin prevented the activation-induced CD4+ T cells from apoptosis. Conclusions: The data indicate that UC CD4+ T cells express high levels of survivin, which impairs the apoptotic machinery in CD4+ T cells and prevents the activation-induced CD4+ T cell apoptosis. Therefore, target therapy against survivin has translational potential in the treatment of UC patients.
- Publication
Journal of Innate Immunity, 2020, Vol 12, Issue 3, p226
- ISSN
1662-811X
- Publication type
Article
- DOI
10.1159/000500546