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- Title
7 Tesla Magnetic Resonance Imaging to Detect Cortical Pathology in Multiple Sclerosis.
- Authors
Yao, Bing; Hametner, Simon; van Gelderen, Peter; Merkle, Hellmuth; Chen, Christina; Lassmann, Hans; Duyn, Jeff H.; Bagnato, Francesca
- Abstract
Background: Neocortical lesions (NLs) are an important pathological component of multiple sclerosis (MS), but their visualization by magnetic resonance imaging (MRI) remains challenging. Objectives: We aimed at assessing the sensitivity of multi echo gradient echo (ME-GRE) T2*-weighted MRI at 7.0 Tesla in depicting NLs compared to myelin and iron staining. Methods: Samples from two MS patients were imaged post mortem using a whole body 7T MRI scanner with a 24-channel receive-only array. Isotropic 200 micron resolution images with varying T2* weighting were reconstructed from the ME-GRE data and converted into R2* maps. Immunohistochemical staining for myelin (proteolipid protein, PLP) and diaminobenzidine-enhanced Turnbull blue staining for iron were performed. Results: Prospective and retrospective sensitivities of MRI for the detection of NLs were 48% and 67% respectively. We observed MRI maps detecting only a small portion of 20 subpial NLs extending over large cortical areas on PLP stainings. No MRI signal changes suggestive of iron accumulation in NLs were observed. Conversely, R2* maps indicated iron loss in NLs, which was confirmed by histological quantification. Conclusions: High-resolution post mortem imaging using R2* and magnitude maps permits detection of focal NLs. However, disclosing extensive subpial demyelination with MRI remains challenging.
- Subjects
MULTIPLE sclerosis research; MAGNETIC resonance imaging; MYELIN; PROTEOLIPIDS; IMMUNOHISTOCHEMISTRY; PATHOLOGY
- Publication
PLoS ONE, 2014, Vol 9, Issue 10, p1
- ISSN
1932-6203
- Publication type
Article
- DOI
10.1371/journal.pone.0108863