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- Title
Telomere capping protein HOAP is phosphorylated via the ATM-Nbs pathway following treatment with DSB inducing drugs in Drosophila.
- Authors
Kinyo On; Yasuko Kato; Masanobu Itoh
- Abstract
The telomere is an indispensable chromatin structure for replication and protection of chromosome ends in eukaryotes. When DNA double-strand-breaks (DSBs) occur, the telomere capping proteins, TRF1 and TRF2, are phosphorylated prior to repair in human cells. Whether a similar phosphorylation of telomere capping proteins occurs in other organisms is not known. To investigate the DSB response of capping proteins in non-human organisms, we used the fruit fly Drosophila, one of the organisms that lacks standard telomeres and telomerase. Our results showed that a Drosophila telomere capping protein, HOAP, was phosphorylated at amino acid (aa) residues 211-270 in response to etoposide. Two DSB repair proteins, Nbs and ATM, were shown to be involved in the phosphorylation of HOAP. These results suggest that HOAP plays a functionally analogous role to mammalian TRF1 and/or TRF2 in the DSB response in Drosophila.
- Subjects
CAPPING proteins; TELOMERES; DROSOPHILA; CHROMOSOME replication; FRUIT flies; CHROMATIN; CELLULAR aging; DNA repair
- Publication
Journal of Insect Biotechnology & Sericology, 2023, Vol 92, Issue 1, p1
- ISSN
1346-8073
- Publication type
Article