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- Title
The VDR and TNF-α gene polymorphisms in Iranian tuberculosis patients: The study on host susceptibility.
- Authors
Varahram, Mohammed; Farnia, Parissa; Anoosheh, Sabar; Kazampour, Mehdi; Merza, Muayad; Saeif, Shima; Masjedi, Mohammad Reza; Velayati, Ali Akbar
- Abstract
Background: Vitamin-D receptor (VDR) and tumor necrosis factor-alpha (TNF-a) genes are thought to be important in the intracellular killing of mycobacteria. This study aimed to determine the association of VDR and TNF-a variant with development of pulmonary tuberculosis (PTB) among Iranian patients. Patients and methods: Selected regions of VDR and TNF-α were amplified, and then the PCR products were digested using restriction enzyme (RFLP). Digested products were run on 8% polyacrylamide gel, and were stained with silver- nitrate. Single nucleotide polymorphisms (SNPs) at restriction sites of BsmI, and FokI of VDR gene and SNPs of TNF-α at -238,-308, -244,-857,-863 positions were analyzed by PCR-RFLP among 117 PTB cases and 60 healthy controls. Results: No statistically significant difference was observed in allele frequencies of FokI of VDR and TNF-α at -238, -244,-863 and -857 position. Although, the frequency of b allele of BsmI (p=0.001) and -308 A variant in TNF-α promoter region (p=0.006) were significantly more in PTB patients than healthy controls. The frequency of extended diplotypes were different in patients and control subjects (p<0.05). Conclusion: This study confirmed the association of VDR BsmI and TNF-α -308A with susceptibility to tuberculosis in Iranian PTB patients. In addition, the results showed the importance of haplotypes and diplotypes analysis in determining the host susceptibility against TB.
- Subjects
VITAMIN D; GENETIC polymorphisms; TUBERCULOSIS patients; DISEASE susceptibility; LUNG diseases; MYCOBACTERIAL diseases; COMMUNICABLE diseases; DNA restriction enzymes; POLYACRYLAMIDE
- Publication
Iranian Journal of Clinical Infectious Diseases, 2009, Vol 4, Issue 4, p207
- ISSN
1735-5109
- Publication type
Article