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- Title
The diagnostic value of gyrB RFLP PCR test in differentiation between pathogenic Mycobacteria in patients with clinical suspicions of tuberculosis in Mazandaran.
- Authors
Nasrollahi, Mohtaram; Bagher, Maryam Pourhaji; Ahanjan, Mohammad; Khalilian, Ali Reza
- Abstract
Background and purpose: Mycobacterium tuberculosis complex (MTBC) members are causative agents of human and animal tuberculosis. Differentiations of MTBC members are required for appropriate treatment of individual patients and reduce the drug resistances. Materials and methods: 1345 samples were collected of patients with clinical suspicions of tuberculosis that referred to health care center of Mazandaran from July 2010 to June 2011. The specimens were stained by the Ziehl-Neelsen staining technique and were cultured on Lowenstein-Jensen medium to detect the mycobacteria. For recognition of Mycobacterium tuberculosis complex were a used MTUB-f and MTUB-r primer (gyrB-PCR1). For differentiation of Mycobacterium tuberculosis complex members were used MTUB-756-Gf and MTUB- 1450Cr (gyrB-PCR2) and RFLP PCR using RsaI restriction enzyme. Results: Of 1345 specimens, only 65(4.83%) isolates were positive culture. Out of 65, 59 (90.76%) were MTBC and 6 (9.24%) identified as Mycobacteria other than tuberculosis. All of 59 isolates were M. tuberculosis and to be showed the typical RsaI pattern. Conclusion: The gyrB-RFLP PCR and using the RsaI restriction enzyme is a rapid and easy technique to perform for differentiation of the members of M. tuberculosis complex and it is useful for rapid treatment of patients and avoids fatilities.
- Subjects
MAZANDARAN (Iran); IRAN; RESTRICTION fragment length polymorphisms; TUBERCULOSIS patients; MYCOBACTERIAL diseases; DIAGNOSTIC examinations; DIAGNOSTIC use of polymerase chain reaction; CLINICAL trials
- Publication
Journal of Mazandaran University of Medical Sciences (JMUMS), 2012, Vol 22, Issue 86, p140
- ISSN
1735-9260
- Publication type
Article