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- Title
Expression of protein kinase C isoforms in renal tissue.
- Authors
Östlund, Eva; Mendez, C. F.; Jacobsson, G.; Fryckstedt, J.; Meister, B.; Aperia, A.
- Abstract
PKC plays a central role for the regulation of renal function. PKC consists of a family of isoenzymes. By employing Northern blot techniques we have demonstrated that mRNA transcripts for the classical Ca2+-dependent, diacylglycerol-activated isoform α, the novel, Ca2+-independent isoform δ and the atypical isoform ζ are abundantly expressed in the rat kidney. The novel PKC-ε was weakly expressed. The classical PKCs βI, βII and γ could not be detected. The mRNA expression of PKC-δ and -ζ increased with age. The intrarenal localization of PKC-α, -δ and -ζ isoforms were studied in the adult kidney using in situ hybridization. In the cortex, the PKC-α isoform showed the strongest hybridization signal. PKC α, β and ζ were all distributed in the outer medulla. The PKC-α probe detected particularly strong signal in the outer stripe of the outer medulla. Western blot confirmed the presence of the PKC-α, -δ and -ζ enzymes in renal tissue. The results show cell-specific and developmentally-dependent expression of three types of PKC isoforms with different responses to diacylglycerol and calcium. The developmental increase of both PKC-δ and PKC-ζ suggests a specific role for these isoforms for the functional regulation of the mature kidney.
- Subjects
PROTEIN kinase C; ISOENZYMES; MESSENGER RNA; GENETIC transcription; TISSUES; KIDNEYS; LABORATORY rats
- Publication
Kidney International, 1995, Vol 47, Issue 3, p766
- ISSN
0085-2538
- Publication type
Article
- DOI
10.1038/ki.1995.117