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- Title
Microinjection of Salusin-β into the Nucleus Tractus Solitarii Inhibits Cardiovascular Function by Suppressing Presympathetic Neurons in Rostral Ventrolateral Medulla in Rats.
- Authors
LU, Y.; WU, Y. S.; CHEN, D. S.; WANG, M. M.; WANG, W. Z.; YUAN, W. J.
- Abstract
Salusin-β is newly identified bioactive peptide of 20 amino acids, which is widely distributed in hematopoietic system, endocrine system, and the central nervous system (CNS). Although salusinβ extensively expressed in the CNS, the central cardiovascular functions of salusin-β are unclear. Our main objective was to determine the cardiovascular effect of microinjection of salusin-β into the nucleus tractus solitarii (NTS) in anesthetized rats. Bilateral or unilateral microinjection of salusin-β (0.94-94 pg/rat) into the NTS dose-dependently decreased blood pressure and heart rate. Bilateral NTS microinjection of salusin-β (9.4 pg/rat) did not alter baroreflex sensitivity. Prior application of the glutamate receptor antagonist kynurenic acid (0.19 pg/rat, n=9) into the NTS did not alter the salusin-β (9.4 pg/rat) induced hypotension and bradycardia. However, pretreatment with the GABA receptor agonist muscimol (0.5 ng/rat) within the rostral ventrolateral medulla (RVLM) completely abolished the hypotension (-14±5 vs. -3±5 mm Hg, P<0.05) and bradycardia (-22±6 vs. -6±5 bpm, P<0.05) evoked by intra-NTS salusin-β (9.4 pg/rat). In addition, we found that vagotomy didn't influence the actions of salusin-β (9.4 pg/rat) in the NTS. In conclusion, our present study shows that microinjection of salusin-β into the NTS significantly produces hypotension and bradycardia, presumably by suppressing the activities of presympathetic neurons in the RVLM.
- Subjects
MICROINJECTIONS; SOLITARY nucleus; CARDIOVASCULAR system physiology; NEURAL physiology; MEDULLA oblongata; LABORATORY rats; PHYSIOLOGY
- Publication
Physiological Research, 2015, Vol 64, Issue 2, p161
- ISSN
0862-8408
- Publication type
Article
- DOI
10.33549/physiolres.932616