We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Molecular characterization of Vitellogenin-like1 gene in Sogatella furcifera (Hemiptera: Delphacidae), and its function on reproduction.
- Authors
Xu, Changyu; Li, Fei; Hou, Maolin; Liu, Yudi
- Abstract
In this study, a vitellogenin like1 gene (SfVg- like1) in Sogatella furcifera was identified. The open reading frame (ORF) encoded 1,321 amino acid sequence. Structure analysis reveals that the amino acid sequence of SfVg- like1 has 3 conserved LPD_N, DUF1943 and VWFD domains. Phylogenetic analyses showed that SfVg- like1 was clustered in the same branch with the Vg- like1 of Nilaparvata lugens (100% bootstrap value) compared with other Hemiptera insects Vg s associated with vitellogenesis. Temporo-spatial expression analyses showed that SfVg -like1 expressed during all stages, and in both genders. The relative expression levels of SfVg -like1 mRNA were higher in adults than in nymph developmental stages. The knockdown of SfVg- like1 gene resulted in the inhibition of the ovarian development in female adults, whereas the morphology of the testis in male adults was not been affected. The silence of SfVg- like1 could decrease the relative expression levels of target of rapamycin (SfTOR , GenBank MW193765) and vitellogenin (SfVg , GenBank MH271114) genes significantly in female adults. However, the knockdown of SfTOR or SfVg genes in female adults did not affect the transcript level of SfVg- like1. Therefore, it demonstrated that SfVg- like1 might locate on the upstream signaling pathways of SfTOR and SfVg. These results demonstrate that SfVg- like1 is essential for S. furcifera reproduction, and it could be the potential target for the control of this pest.
- Subjects
GENE expression; NILAPARVATA lugens; HEMIPTERA; GENES; PEST control
- Publication
Journal of Insect Science, 2024, Vol 24, Issue 1, p1
- ISSN
1536-2442
- Publication type
Article
- DOI
10.1093/jisesa/ieae013