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- Title
Independent validation of experimental results requires timely and unrestricted access to animal models and reagents.
- Authors
Diegel, Cassandra R.; Hann, Steven; Ayturk, Ugur M.; Hu, Jennifer C. W.; Lim, Kyung-Eun; Droscha, Casey J.; Madaj, Zachary B.; Foxa, Gabrielle E.; Izaguirre, Isaac; Robling, Alexander G.; Warman, Matthew L.; Williams, Bart O.
- Abstract
We used CRISPR/Cas9 gene editing to create mice that are lacking I Bglap i and I Bglap2 i , which encode osteocalcin [[1]]. Furthermore, the osteocalcin-null rat model did not develop obesity, insulin resistance, or glucose intolerance, which conflicts with Dr. Karsenty's mice [[6]]. Should Dr. Karsenty make available batches of his biologically-active osteocalcin without restriction, interested parties could avoid the potential confounder of reagent quality [[8]] and assess objectively whether osteocalcin has a hormonal role when administered exogenously.
- Subjects
ANIMAL models in research; CELL receptors; BONES; BONE mechanics; CANCELLOUS bone
- Publication
PLoS Genetics, 2020, Vol 16, Issue 6, p1
- ISSN
1553-7390
- Publication type
Article
- DOI
10.1371/journal.pgen.1008940