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- Title
Isolation and characterization of ten novel microsatellite loci in the red-winged tinamou ( Rhynchotus rufescens, Tinamiformes, Aves) and cross-amplification in other tinamous.
- Authors
Santos, Dimas; Moreira, Lucas; Tonhati, Humberto; Caparroz, Renato
- Abstract
We describe the isolation and characterization of ten microsatellite loci from the red-winged tinamou ( Rhynchotus rufescens) and also evaluated the cross-amplification of these loci and other ten loci previously developed for the great tinamou ( Tinamus major) in other tinamous. Genetic variability was assessed using 24 individuals. Six loci were polymorphic with moderate to high number of alleles per locus (2-12 alleles) and showed expected heterozygosity (HE) ranging from 0.267 to 0.860. All loci conformed to the Hardy-Weinberg expectation and linkage disequilibrium was not significant for any pair of loci. This battery of polymorphic loci showed high paternity exclusion probability (0.986) and low genetic identity probability (4.95 × 10), proving to be helpful for parentage tests and population analyses in the red-winged tinamou. The cross-amplification was moderate where of the 160 locus/taxon combinations, 46 (28.75%) successfully amplified.
- Subjects
MICROSATELLITE repeats; RHYNCHOTUS rufescens; GENETIC polymorphisms; GENE amplification; RHYNCHOTUS; TINAMIFORMES
- Publication
Molecular Biology Reports, 2012, Vol 39, Issue 4, p4837
- ISSN
0301-4851
- Publication type
Article
- DOI
10.1007/s11033-011-1277-1