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- Title
Effect of shRNA‐mediated Xist knockdown on the quality of porcine parthenogenetic embryos.
- Authors
Chen, Xiaoyu; Zhu, Zhiwei; Yu, Fuxian; Huang, Jing; Jia, Ruoxin; Pan, Jianzhi
- Abstract
Background: Parthenogenetically activated oocytes exhibit poor embryo development and lower total numbers of cells per blastocyst accompanied by abnormally increased expression of Xist, a long noncoding RNA that plays an important role in triggering X chromosome inactivation during embryogenesis. Results: To investigate whether knockdown of Xist influences parthenogenetic development in pigs. We developed an anti‐Xist short hairpin RNA (shRNA) vector, which can significantly inhibit Xist expression for at least seven days when injected at 12–13 hr after parthenogenetic activation. Embryonic cleavage, blastocyst formation, and total blastocyst cell numbers were compared during the blastocyst stage, as well as the expression of an X‐linked gene and three pluripotent transcription factors. Knockdown of Xist significantly increases the total blastocyst cell number, but does not influence the rate of embryo cleavage and blastocyst formation. The expressions of Sox2, Nanog, and Oct4 were also significantly improved in the injected embryos compared with the control at the blastocyst stage, but the Foxp3 expression level was not increased significantly. Conclusions: The present study provides valuable information for understanding the role of Xist in parthenogenesis and presents a new approach for improving the quality of porcine parthenogenetic embryos. Developmental Dynamics 248:140–148, 2019. © 2018 Wiley Periodicals, Inc. Key Findings: We developed one shRNA vector to inhibit Xist expression significantly in embryos.Knockdown of Xist improved total cell number of parthenogenetic blastocyst.Knockdown of Xist upregulated Sox2, Nanog and Oct4 in parthenogenetic blastocyst.
- Publication
Developmental Dynamics, 2019, Vol 248, Issue 1, p140
- ISSN
1058-8388
- Publication type
Article
- DOI
10.1002/dvdy.24660