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- Title
In vitro functional response of human tendon cells to different dosages of low-frequency pulsed electromagnetic field.
- Authors
Girolamo, L.; Viganò, M.; Galliera, E.; Stanco, D.; Setti, S.; Marazzi, M.; Thiebat, G.; Corsi Romanelli, M.; Sansone, V.; de Girolamo, L; Viganò, M; Marazzi, M G; Corsi Romanelli, M M
- Abstract
<bold>Purpose: </bold>Chronic tendinopathy is a degenerative process causing pain and disability. Current treatments include biophysical therapies, such as pulsed electromagnetic fields (PEMF). The aim of this study was to compare, for the first time, the functional in vitro response of human tendon cells to different dosages of PEMF, varying in field intensity and duration and number of exposures.<bold>Methods: </bold>Tendon cells, isolated from human semitendinosus and gracilis tendons (hTCs; n = 6), were exposed to different PEMF treatments (1.5 or 3 mT for 8 or 12 h, single or repeated treatments). Scleraxis (SCX), COL1A1, COL3A1 and vascular endothelial growth factor-A (VEGF-A) expression and cytokine production were assessed.<bold>Results: </bold>None of the different dosages provoked apoptotic events. Proliferation of hTCs was enhanced by all treatments, whereas only 3 mT-PEMF treatment increased cell viability. However, the single 1.5 mT-PEMF treatment elicited the highest up-regulation of SCX, VEGF-A and COL1A1 expression, and it significantly reduced COL3A1 expression with respect to untreated cells. The treated hTCs showed a significantly higher release of IL-1β, IL-6, IL-10 and TGF-β. Interestingly, the repeated 1.5 mT-PEMF significantly further increased IL-10 production.<bold>Conclusions: </bold>1.5 mT-PEMF treatment was able to give the best results in in vitro healthy human tendon cell culture. Although the clinical relevance is not direct, this investigation should be considered an attempt to clarify the effect of different PEMF protocols on tendon cells, in particular focusing on the potential applicability of this cell source for regenerative medicine purpose, both in surgical and in conservative treatment for tendon disorders.
- Subjects
PROTEIN metabolism; BIOCHEMISTRY; CELL culture; CELL physiology; COLLAGEN; ELECTROMAGNETIC fields; GROWTH factors; INTERLEUKINS; PHENOMENOLOGY; TENDONS; VASCULAR endothelial growth factors; METABOLISM
- Publication
Knee Surgery, Sports Traumatology, Arthroscopy, 2015, Vol 23, Issue 11, p3443
- ISSN
0942-2056
- Publication type
journal article
- DOI
10.1007/s00167-014-3143-x