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- Title
CryoET of Single Particle CryoEM Grids Reveals Widespread Particle Adsorption to the Air-Water Interface, Which May be Reduced with New Plunging Techniques.
- Authors
Noble, Alex J.; Dandey, Venkata P.; Wei, Hui; Brasch, Julia; Chase, Jillian; Acharya, Priyamvada; Tan, Yong Zi; Zhang, Zhening; Kim, Laura Y.; Scapin, Giovanna; Rapp, Micah; Eng, Edward T.; Rice, William J.; Cheng, Anchi; Negro, Carl J.; Shapiro, Lawrence; Peter D.; Kwong; Jeruzalmi, David; des Georges, Amedee
- Abstract
The article focuses on cryo-electron tomography (cryoET) revealing that the majority of particles in single particle cryo-electron microscopy (cryoEM) samples are adsorbed to the air-water interfaces during vitrification, and presents a method using Spotiton with varying sample application.
- Subjects
AIR-water interfaces; ADSORPTION (Chemistry); INSULIN receptors; MOLECULAR biology
- Publication
Microscopy & Microanalysis, 2019, p872
- ISSN
1431-9276
- Publication type
Abstract
- DOI
10.1017/S1431927618004853