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- Title
Comparison of betanodavirus replication efficiency in ten Indian fish cell lines.
- Authors
Sarath Babu, V.; Abdul Majeed, S.; Nambi, K.; Taju, G.; Madan, N.; Sundar Raj, N.; Sahul Hameed, A.
- Abstract
Ten cell lines established from Indian marine, brackishwater and freshwater fish were tested for their susceptibility to fish nodavirus. In addition, the efficiency of betanodavirus replication was tested in these cell lines. Multiple vacuolation, a typical cytopathic effect for virus infection, was observed in infected SISK, SISS, SIGE and ICF cells. Infection of the different fish cell lines was confirmed by RT-PCR, immunodot blot assay and indirect ELISA. The virus concentration in culture supernatant collected from infected sea bass and grouper cell lines increased progressively from 10 at day 1 postinfection to 10 TCID ml at day 9. The amount of virus in different cell lines was also quantified by real-time PCR. These results indicate the suitability of the SISK, SISS, and SIGE cell lines for fish nodavirus propagation for developing viral diagnostics and vaccines.
- Subjects
CELL lines; DISEASE susceptibility; NODAVIRUSES; VIRUS diseases; ENZYME-linked immunosorbent assay; REVERSE transcriptase polymerase chain reaction; VIRAL replication
- Publication
Archives of Virology, 2013, Vol 158, Issue 6, p1367
- ISSN
0304-8608
- Publication type
Report
- DOI
10.1007/s00705-013-1617-7