We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Alteration of the Transcriptional Profile of Human Embryonic Kidney Cells by Transient Overexpression of Mouse TRPM7 Channels.
- Authors
Lee, Byoung-Cheol; Hong, Seong-Eui; Lim, Hyun-Ho; Kim, Do Han; Park, Chul-Seung
- Abstract
Background: TRPM7 is a cation channel containing a functional kinase domain. The functional activity of TRPM7 is essential for cell viability and growth, and its expression is up-regulated in certain pathological conditions, such as ischemia. Methods: In order to assess the effects of TRPM7 activity on cellular gene expression, inducible HEK293 cell-lines harboring the wild-type mouse TRPM7 and a mutant lacking the kinase domain were established. The wild-type and the non-functional TRPM7 channels were induced transiently and the comparative changes in cellular transcription were investigated. Results: From the genome-scale analysis using a human genome expression microarray, we identified 951 genes altered in transcription significantly and specifically by the expression of the functional TRPM7 channel. Conclusion: By analyzing the genes differentially expressed by TRPM7, we were able to provide potential target proteins and to delineate the cellular pathways affected by the channel function. Copyright © 2011 S. Karger AG, Basel
- Subjects
TRANSCRIPTION factors; GENE expression; TRP channels; KIDNEY diseases; GROWTH factors; ISCHEMIA; PROTEIN microarrays; LABORATORY mice
- Publication
Cellular Physiology & Biochemistry (Karger AG), 2011, Vol 27, Issue 3/4, p313
- ISSN
1015-8987
- Publication type
Article
- DOI
10.1159/000327958