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- Title
CRISPRcloud: a secure cloud-based pipeline for CRISPR pooled screen deconvolution.
- Authors
Hyun-Hwan Jeong; Seon Young Kim; Rousseaux, Maxime W. C.; Zoghbi, Huda Y.; Zhandong Liu
- Abstract
Summary: We present a user-friendly, cloud-based, data analysis pipeline for the deconvolution of pooled screening data. This tool, CRISPRcloud, serves a dual purpose of extracting, clustering and analyzing raw next generation sequencing files derived from pooled screening experiments while at the same time presenting them in a user-friendly way on a secure web-based platform. Moreover, CRISPRcloud serves as a useful web-based analysis pipeline for reanalysis of pooled CRISPR screening datasets. Taken together, the framework described in this study is expected to accelerate development of web-based bioinformatics tool for handling all studies which include next generation sequencing data.
- Subjects
CRISPRS; NUCLEOTIDE sequence; LOCUS (Genetics); DECONVOLUTION of absorption spectra; BIOINFORMATICS
- Publication
Bioinformatics, 2017, Vol 33, Issue 18, p2963
- ISSN
1367-4803
- Publication type
Article
- DOI
10.1093/bioinformatics/btx335