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- Title
Homocysteine influences the human keratocytes cell cycle.
- Authors
Paduch, Roman; Matysik-Woźniak, Anna; Flis, Beata; Jünemann, Anselm G.; Rejdak, Robert
- Abstract
BACKGROUND: Homocysteine (Hcy), a metabolic intermediate, is a sulfur-containing amino acid not present in the structure of proteins. It has been shown that high Hcy levels via oxidative stress, induced inflammation, and vessel dysfunction may affect the functioning of tissues, including the structures that form the eye, among others keratocytes. The visual disturbance caused by high levels of Hcy may also be associated with disturbed cell proliferation resulting from the effect of this amino acid on the cell cycle. The goal was to analyse the influence of Hcy on the keratocytes and to find out in which phase of the cell cycle its course is disturbed by this amino acid. MATERIALS AND METHODS: A normal human keratocytes (HK) cell line was used in the study. May-Grünwald-Giemsa (MGG) staining for morphology visualization and cytometric cell cycle analysis were performed. RESULTS: Hcy does not affect the G1 phase of the cycle, while it regulates the S and G2 phases. Changes in the amount of the sub-G1 population indicative of a pro-apoptotic effect of Hcy on keratocytes were detected. The form of the Hcy administered (L stereoisomer or DL racemic mixture) and the amino acid concentration were also important. CONCLUSIONS: Homocysteine influences the keratocyte cell cycle. The change occurs at the stage of the G1-S transition, which suggests a decreasing level of cells in the S phase and an increasing level in the G2 phase. The long-term influence of Hcy on keratocytes may affect keratocytes proliferation and possible cornea regeneration.
- Subjects
HOMOCYSTEINE; ANTIBIOTICS; FLOW cytometry; PHENOMENOLOGICAL biology; CORNEA; DATA analysis; CELL physiology; CELL proliferation; CELL cycle; BIOCHEMISTRY; DESCRIPTIVE statistics; FIBROBLASTS; CELL lines; EXPERIMENTAL design; AMINO acids; ANALYSIS of variance; STATISTICS; STAINS &; staining (Microscopy); DATA analysis software; BIOMARKERS; PHYSIOLOGY
- Publication
Ophthalmology Journal (2450-7873), 2023, Vol 8, p81
- ISSN
2450-7873
- Publication type
Article
- DOI
10.5603/OJ.2023.0017