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- Title
Rapid and stable production of 2,3-butanediol by an engineered <italic>Saccharomyces cerevisiae</italic> strain in a continuous airlift bioreactor.
- Authors
Yamada, Ryosuke; Nishikawa, Riru; Wakita, Kazuki; Ogino, Hiroyasu
- Abstract
Utilization of renewable feedstocks for the production of bio-based bulk chemicals, such as 2,3-butanediol (2,3-BDO), by engineered strains of the non-pathogenic yeast, <italic>Saccharomyces cerevisiae</italic>, has recently become an attractive option. In this study, to realize rapid production of 2,3-BDO, a flocculent, 2,3-BDO-producing <italic>S. cerevisiae</italic> strain YPH499/dPdAdG/BDN6-10/FLO1 was constructed from a previously developed 2,3-BDO-producing strain. Continuous 2,3-BDO fermentation was carried out by the flocculent strain in an airlift bioreactor. The strain consumed more than 90 g/L of glucose, which corresponded to 90% of the input, and stably produced more than 30 g/L of 2,3-BDO over 380 h. The maximum 2,3-BDO productivity was 7.64 g/L/h at a dilution rate of 0.200/h, which was higher than the values achieved by continuous fermentation using pathogenic bacteria in the previous reports. These results demonstrate that continuous 2,3-BDO fermentation with flocculent 2,3-BDO-producing <italic>S. cerevisiae</italic> is a promising strategy for practical 2,3-BDO production.
- Subjects
BUTANEDIOL manufacturing; SACCHAROMYCES cerevisiae; AIRLIFT bioreactors; FEEDSTOCK; FUNGAL biotechnology
- Publication
Journal of Industrial Microbiology & Biotechnology, 2018, Vol 45, Issue 5, p305
- ISSN
1367-5435
- Publication type
Article
- DOI
10.1007/s10295-018-2033-5